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. 2021 Apr 19;7(6):1607–1618. doi: 10.1021/acsinfecdis.0c00774

Figure 3.

Figure 3

Amp-LPN mediates the clearance of E. faecalis. (A) The T. pyriformis was infected by E. faecalis at OD 0.125 2 h prior to the free Amp and Amp-LPN treatments (early intervention). (B) The E. faecalis cell count (CFU/mL) and (C) the T. pyriformis survival count were assessed 3 and 24 h post infection treatment (hpit). (D) The T. pyriformis was infected by E. faecalis at OD 0.125 8 h prior to the free Amp and Amp-LPN treatments (late intervention). (E) The E. faecalis cell count (CFU/mL) and (F) the T. pyriformis survival count were assessed 20 and 40 h postinfection. Multiple t tests with Holm-Sidak method were performed to investigate the effect of LPN encapsulation. One-way ANOVA test with Dunnett’s multiple comparison was performed for free Amp groups at 3 hpit. The statistical differences are indicated as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns stands for nonsignificant. The results shown are representatives of three independent experiments (Mean ± SD, n = 6).