TABLE 2.

Accuracy, within-run and between-run precision for Indo5 in rat plasma and tissues.

Matrix	QC level	Nominal concentration (ng/ml)	Accuracy (%)	Precision (CVa %)
				Within-run (n = 5)	Between-run (n = 5)
Plasma	LLOQb	1	88.3	4.3	14
	LOW	5	92	7.9	11.7
	MEDIUM	100	90	6.6	8.4
	HIGH	500	97	2	5.2
Liver	LLOQ	1	86.1	12.4	13.2
	LOW	5	105.8	1.2	9.1
	MEDIUM	100	94.4	2	4.8
	HIGH	500	101.2	3.4	6
Kidney	LLOQ	1	110	5.4	14.6
	LOW	5	103	6.8	3.6
	MEDIUM	100	98.4	7.4	13.9
	HIGH	500	102.7	2.6	3.9
Heart	LLOQ	1	86.5	12.7	12.9
	LOW	5	106.2	9.4	11.7
	MEDIUM	100	111.3	4.7	6.4
	HIGH	500	97.9	2.7	4.1
Lung	LLOQ	1	85.8	12.7	12.8
	LOW	5	100.5	7.8	10.9
	MEDIUM	100	93.2	10.4	11.6
	HIGH	500	97.2	6.3	9.5
Stomach	LLOQ	1	85.5	14.3	13.9
	LOW	5	107.7	6.1	14.1
	MEDIUM	100	107.4	6	7.5
	HIGH	500	101.7	8.6	3.2
Small intestine	LLOQ	1	112.4	9.7	14.7
	LOW	5	94.6	2.6	6.5
	MEDIUM	100	104.2	3.1	3.7
	HIGH	500	102.9	2.5	3.9
Large intestine	LLOQ	1	113.5	4.1	9.3
	LOW	5	91.4	2.7	8.9
	MEDIUM	100	98.3	4.6	10.9
	HIGH	500	106.6	3.8	6.5
Spleen	LLOQ	1	87.6	10.3	9.6
	LOW	5	91.3	3.5	3.9
	MEDIUM	100	94.2	2.7	10.1
	HIGH	500	105.9	3.3	7.5
Brain	LLOQ	1	88.6	12.8	12.5
	LOW	5	112.1	8	11.5
	MEDIUM	100	95.4	3.3	10.7
	HIGH	500	101	4.3	5.1
Testis	LLOQ	1	111.8	8.8	13.9
	LOW	5	99	3.6	9.8
	MEDIUM	100	104.3	8.5	10.3
	HIGH	500	102.9	3.2	2.4
Small-intestine content	LLOQ	1	111	14.7	12.2
	LOW	5	92.7	7.2	10.4
	MEDIUM	100	106.4	2.4	2.9
	HIGH	500	98.3	4	3.7
Large-intestine content	LLOQ	1	89.2	13.2	13.9
	LOW	5	93.6	8.6	10.9
	MEDIUM	100	106.2	3.3	9
	HIGH	500	102.2	4.7	6.2

^aCV:coefficient of variation.

^bLLOQ:lower limit of quantification.