Table 1.
Overview and comparison of CBL genes in S. bicolor and S. spontaneum
| S. bicolor | S. spontaneum | Similaritye | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene | Chr.a | Aab | No. of exon | Mwc (kDa) | P.L.d | Gene | Clade | Chr.a | Aab | No. of exon | Mwc (kDa) | P.L.d | |
| Sobic.001G294300 | 1 | 213 | 8 | 25.11 | PM | SsCBL01 | III | 1 | 213 | 8 | 24.49 | PM | 100.00% |
| Sobic.008G152800 | 8 | 225 | 8 | 25.84 | PM/Tono | SsCBL02 | IV | 2 | 225 | 8 | 25.84 | PM/Tono | 97.35% |
| NA | NA | NA | NA | NA | NA | SsCBL03 | IV | 1 | 225 | 8 | 25.77 | PM/Tono | NA |
| Sobic.009G210300 | 9 | 212 | 8 | 24.05 | PM | SsCBL04 | I | 7 | 211 | 8 | 24.11 | PM/Cyto | 78.54% |
| Sobic.003G208400 | 3 | 218 | 8 | 25.82 | PM/Cyto | SsCBL05 | I | 3 | 220 | 8 | 25.66 | PM/Cyto | 96.98% |
| Sobic.008G046500 | 8 | 223 | 8 | 25.68 | PM | SsCBL06 | IV | 7 | 223 | 8 | 25.68 | PM | 99.11% |
| Sobic.004G130600 | 4 | 213 | 8 | 24.39 | PM/Cyto | SsCBL08 | I | 4 | 213 | 8 | 24.34 | PM/Cyto | 99.07% |
| Sobic.003G196400 | 3 | 319 | 9 | 35.87 | PM | SsCBL09 | II | 3 | 294 | 9 | 33.11 | PM | 94.59% |
| Sobic.003G275000 | 3 | 283 | 8 | 32.29 | PM/Tono | SsCBL10 | II | 3 | 288 | 8 | 32.4 | PM/Tono | 87.59% |
PM plasma membrane, Tono tonoplast, Cyto cytoplasm.
aChromosome carrying the target gene
bNumber of amino acids of CBL protein sequence
cPrediction of Molecular weight (Mw) through ExPASy (https://web.expasy.org/compute_pi/)
dSubcellular location of the HAK proteins predicted with WoLF PSORT (https://www.genscript.com/wolf-psort.html)
eSimilarity of protein sequence between S. spontaneum and sorghum determined by BLASTP