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Identification of bi-allelic HFM1 variants in two Chinese azoospermic men. A-B The two families affected by the variants in HFM1. The red frames indicate mutated positions in the Sanger sequencing results. C The affected amino acid residues of HFM1 are conserved among species. The red arrows indicate the locations of HFM1 variants occurring in the domains of HFM1 protein. M, HFM1 mutation; WT, wild type
