Fig. 2.

Expression of 5A and 5N in the human cortex across development. A The expression of 5A (red) and 5N (blue) in SCN1A is shown for 176 BrainVar human cortex (DLPFC) samples across development (points). On the left, the colored line shows the Loess smoothed average and 95% confidence interval (shaded region). On the right, boxplots show the median and interquartile range for the same data, binned into fetal, transitional, and mature developmental stages. B The ratio of 5A and 5N expression from panel A is shown across development (left) and in three developmental stages (right). C–H Panels A and B are repeated for the genes SCN2A, SCN3A, SCN8A. For comparison, the 5A/5N ratio is shown on the same y-axis in Additional file 1: Fig. S3, and equivalent plots for CDS four and six are shown in Additional file 1: Fig. S4. CPM: Counts per million; DLPFC: Dorsolateral prefrontal cortex. Statistical tests: B, D, F, H Left panel, linear regression of log₂(5A:5N ratio) and log₂(post-conceptual days). Right panel, two-tailed Wilcoxon test of log₂(5A:5N ratio) values between fetal and mature groups