Skip to main content
. Author manuscript; available in PMC: 2021 Aug 24.
Published in final edited form as: New Phytol. 2021 Feb 18;230(6):2292–2310. doi: 10.1111/nph.17202

Fig. 5.

Fig. 5

The cytosolic pH in guard cells (GCs) – important factor for stoma movement. Time-lapse CapHensor imaging in GCs of Nicotiana tabacum epidermal strips together with stomata aperture monitoring. Mean [Ca2+]cyt ratio (red), [H+]cyt ratio (green) and stomata aperture area (black) over time. (a) Simultaneous application of 3 mM BTA and 10 μM ABA followed by exclusive ABA treatment (n = 22). (b) 10 μM ABA-pretreated GCs were challenged with 3 mM BTA plus 10 μM ABA followed by treatment with 10 μM ABA only (n = 22). (c) Simultaneous application of 3 mM BTA together with 1 μM flg22 followed by exclusive flg22 treatment (n = 24). (d) Increase of the medium Ca2+-concentration via perfusion from 10 μM CaCl2 to 1 mM CaCl2 (n = 22) and back. Error bars = SE.