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. 2021 Aug 10;12:713704. doi: 10.3389/fimmu.2021.713704

Figure 3.

Figure 3

IgG Immune Complexes Suppress IFN-γ Secretion by Activated Naïve CD8+ T cells and Inhibit Effector Molecule Production by Cytotoxic Memory T Cells (A–C) IFN- γ, IL-2, and IL-5 secretion by activated naïve CD8+ T cells incubated with (WT) IgG1-ICs or controls (Fc5 IgG1-ICs, monomeric IgG1, or PBS). Dashed line represents the average cytokine concentration in non-activated cultures from two donors (D) Flow cytometry analysis of intracellular production of perforin (Prf) and granzyme-β (Gzmβ) in non-proliferating effector memory T cells. Purified CCR7- T cells were either (i) unstimulated and untreated or (ii) activated and incubated with IgG1-ICs or indicated controls. The percent of Gzmβ(-) Prf(-) cells is indicated in blue. (E) Gzmβ/Prf production as a function of IgG1-IC concentration or 50μg/mL negative controls. The assay was run in triplicate wells. (A–E) Error bars represent the standard error of the mean (SEM). Asterisks *, **, and *** indicate p-values < 0.05, p-values < 0.01, and p-values < 0.0001 respectively. “n.s.” stands for statistically not significant.