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. 2021 Aug 10;12:656663. doi: 10.3389/fimmu.2021.656663

Figure 6.

Figure 6

IP-10 activates LIMK–cofilin signaling in resting memory CD4+ T cells. (A) Gating strategy for the experiment evaluating the effect of IP-10 on cofilin activation. (B, C) Representative flow cytometry plots from the experiment evaluating the effect of IP-10 on cofilin phosphorylation over time. Resting memory CD4+ T cells were treated with IP-10 (5 ng/ml) and IP-10 (5 ng/ml)+R10015 (100 μM) (B) or NL4-3 and NL4-3+IP-10 (5 ng/ml) (C) for the indicated times, and p-cofilin was marked with Ser3, followed by flow cytometry analysis. The red dashed lines represent localized baselines for cofilin phosphorylation. Cofilin dynamics were evaluated at 1, 5, 10, 15, 30, 45, and 60 min, with the measurement at 0 min serving as the reference value. (D, E) LIMK inhibition abrogates the enhancement of HIV AD8/NL4-3 infection by IP-10. Representative plots of 2 participants are shown. P24 levels of day 14 were compared between groups by one-way analysis of variance (NL4-3: n = 7, AD8: n = 8). *P < 0.05.