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. 2021 Aug 4;22(4):1123. doi: 10.3892/etm.2021.10557

Figure 4.

Figure 4

Combination treatment with AST IV and PNS suppresses pyroptosis in rats with cerebral ischemia-reperfusion. (A) Western blot analysis of pyroptosis-associated proteins (n=5). β-actin was used as the internal reference. Quantification of (B) NLRP3, (C) ASC, (D) caspase-1, (E) IL-1β, (F) IL-18, (G) GSDMD and (H) GSDMD-N protein expression levels (n=5). The groups are as follows: i) Sham, rats with sham operation; ii) model, rats with MCAO; iii) AST IV, rats with MCAO + 28 mg/kg AST IV; iv) PNS, rats with MCAO + 80 mg/kg PNS; and v) combination, rats with MCAO + 28 mg/kg AST IV + 80 mg/kg PNS. **P<0.01 vs. Sham; #P<0.05, ##P<0.01 vs. Model; ΔP<0.05, ΔΔP<0.01 vs. AST IV or PNS. ASC, apoptosis-associated speck-like protein containing a caspase activation and recruitment domain; AST IV, astragaloside IV; GSDMD, gasdermin D; MCAO, middle cerebral artery occlusion; N, N-terminal; NLRP3, nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3; PNS, Panax notoginseng saponins.