Figure 5.

PEVs activate the CXCR2-mediated NF-κB pathway in RA-FLS. (A) Western blotting was performed to detect the expression levels of IκB, p-IκB, NF-κB and p-NF-κB in RA-FLS following treatment with different concentrations of SB225002, with or without PEVs. Representative blots of three independent experiments are demonstrated and were normalized against GAPDH. (B) Semi-quantification of fold change in p-IκB relative to total IκB and p-NF-κB relative to total NF-κB are presented as the mean ± SD. ^*P<0.05 vs. the control group; ^#P<0.05 vs. 50 µg/ml PEVs group. PEVs, platelet-derived extracellular vesicles; NF, nuclear factor; RA-FLS, rheumatoid arthritis fibroblast-like synoviocytes; p, phosphorylated; CXCR2, C-X-C motif chemokine receptor 2.