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. 2021 Aug 19;153(10):e202112959. doi: 10.1085/jgp.202112959

Figure 1.

Figure 1.

Method of nonstationary fluctuation (noise) analysis of MET currents in a P4 basal OHC. (A) Mean MET current in response to a 30-Hz sinusoidal fluid jet deflection of the hair bundle. 40 traces averaged. (B) Mean variance of the MET current determined by subtracting the mean current from each of the responses, squaring, and averaging (red on the rising phase and blue on the falling phase of the current). The variance is maximal when the channel open probability is ∼0.5. The current variance is abolished by perfusion of 0.2 mM of the MET channel blocker dihydrostreptomycin. (C) Plot of the variance against the mean current for the rising phase (red) and the falling phase (blue) of the current. Smooth parabolas are fits with Eq. 1 to give values for the single-channel current (red, i = −3.8 pA; blue, i = −3.4 pA). (D) Variance for the rising phase (segment from 0 to 16.7 ms) was averaged with variance of the falling phase (segment from 33.3 to 16.7 ms) and plotted versus mean current. Red line is fit to Eq. 1, with i = −3.7 pA and NMET = 322 channels. Holding potential, −84 mV.