Figure 2.
Comparison of noise analysis and single-channel events in a P4 apical OHC of a Tmc1+/+; Tmc2−/− mouse. (A) Examples of three channel events for bundle deflection with ensemble average of 35 stimuli shown below. Red dashed lines correspond to estimate of channel size in each trace. (B) Amplitude histograms of the three recordings in A, with two Gaussian fits with peak currents denoted on each panel. (C) Response to a half cycle of sinusoidal bundle deflection showing the mean current (bottom) and the current variance (top). (D) Plot of variance (average of the rising and falling phases) against mean of current; smooth red line is fit with Eq. 1 to give i = −3.5 pA and NMET = 122 channels. Noise analysis was performed first, and BAPTA was then applied to obtain single-channel events. Holding potential, −84 mV. Var, variance.