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. 2021 Aug 20;218(10):e20200464. doi: 10.1084/jem.20200464

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© 2021 Lindsay et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 1. — CD11c⁺ cells in islets prevent T cell arrest. (A–K) NOD.CD11c-DTR BM chimeras containing 90% NOD.CD11c-DTR BM + 10% NOD.CD11c-DTR.8.3.CD2-dsRed BM (A–G) or 95% CD11c-DTR + 5% CD11c-DTR.CD2-dsRed (H–K) were treated twice with PBS (control) or DT (CD11c depleted). (A and H) Schematic of experimental setup to analyze T cell motility in explanted islets by two-photon microscopy. (B) Average level of islet infiltration. (C and I) Average T cell crawling speed per islet. (D) Example of representative 15-min tracks of motion of 15 randomly selected T cells from individual islets. (E) MSD over time indicates the T cells’ ability to translocate away from their place of origin, averaged per islet. (F, G, J, and K) Arrest coefficient, or the percentage of time a T cell is moving <1.5 µm/min, averaged per islet. (A–K) Data are pooled from three independent experiments, and error bars represent SEM. (A–G) n = 10 islets containing 647 analyzed 8.3 T cells from PBS treated mice and n = 11 islets containing 371 analyzed 8.3 T cells from DT treated mice. (H–K) n = 13 islets containing 156 analyzed polyclonal T cells from PBS treated mice and n = 15 islets containing 329 analyzed polyclonal T cells from DT treated mice. (B, C, F, I, and J) Statistics: Student's t test. (E, G, and K) Statistics: two-way ANOVA with Bonferroni’s multiple comparison test, treatment effect (black statistic), interaction effect (red statistic). (B–K) *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.