Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 20;218(10):e20200464. doi: 10.1084/jem.20200464

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Lindsay et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — MERTK signaling drives antigen-independent suppression of T cell arrest in islets. Activated 8.3 CD8 T cells, BDC-2.5 CD4 T cells, and polyclonal T cells were fluorescently labeled and cotransferred into female NOD.CD11c-YFP mice. Mice were treated twice daily with saline vehicle or 30 mg/kg UNC2025, a small-molecule inhibitor of MERTK and Flt3. 17 h following the initial treatment, islets were isolated and analyzed by two-photon microscopy. (A) Schematic of experimental setup. (B) Average level of islet infiltration. (C) Average T cell crawling speed per islet. (D and E) Arrest coefficient: percentage of time that a cell crawls <1.5 µm/min, averaged per islet. (F) MSD over time indicates the T cells’ ability to translocate away from their place of origin, averaged per islet. (A–F) n = 17 islets from vehicle-treated mice containing 590 analyzed BDC-2.5 T cells, 182 analyzed 8.3 T cells, and 497 analyzed polyclonal T cells. n = 8–11 islets from UNC2025 treated mice containing 481 analyzed BDC-2.5 T cells, 49 analyzed 8.3 T cells, and 671 analyzed polyclonal T cells. Data were derived from two or three independent experiments. (B–D) Statistics: two-tailed Student’s t test. Error bars represent SEM. (E and F) Statistics: two-way ANOVA with Bonferroni’s multiple comparison test, treatment effect (black statistic), interaction effect (red statistic); *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Error bars represent SEM.