Figure S1.

CD11c-DTR-GFP cells are effectively depleted in the islets and include CD11b⁺, XCR1⁺, and MERTK⁺ populations. NOD.CD11c-DTR-GFP BM chimeras containing NOD.CD11c-DTR-GFP BM were treated twice with PBS or DT. (A and B) Islets from treated NOD.CD11c-DTR BM chimeras (A) or NOD.CD11c-DTR-GFP⁺CD2.dsRed⁺ female mice (B) were isolated, digested and dissociated, stained, and analyzed by flow cytometry. (A) Quantification of CD11c⁺ cells in islets following PBS or DT treatment. (B) Quantification of the composition of the CD11c-DTR-GFP⁺ versus GFP⁻ mononuclear phagocytes in the islets. (A) n = 12 mice (PBS), n = 16 mice (DT) from seven independent experiments. Error bars represent SEM. Statistics: Student’s t test; ***, P < 0.001. (B) n = 4 mice from three independent experiments.