Figure S2.

Zbtb46-expressing DCs are not the subset of CD11c⁺ cells in islets that prevent T cell arrest. (A–D) Islet infiltration was induced in B6.Zbtb46-DTR.RIP-mOva BM chimeras by the transfer of naive OT-I.GFP T cells. (A) 3–5 d after T cell transfer, the chimeras were treated twice with PBS (control) or DT (Zbtb46 depleted). 48 h after treatment initiation, T cell motility was analyzed in explanted islets by two-photon microscopy. (B) Average T cell crawling speed per islet. (C) Arrest coefficient: percentage of time a T cell is moving <1.5 µm/min. (D) Average islet infiltration levels. (E) Flow cytometric analysis of mononuclear phagocytes in the islets of PBS (control)– or DT (Zbtb46-depleted)–treated NOD.Zbtb46-DTR BM chimeras, 48 h after treatment initiation. (A–D) Data were pooled from three independent experiments. n = 10 islets from PBS-treated mice and 26 islets from DT-treated mice. (E) Data were pooled from four or five mice per condition in two independent experiments. (B, D, and E) Statistics: Student’s t test. (C) Statistics: two-way ANOVA. Error bars represent SEM. (B–E) Error bars represent SEM. *, P < 0.05.