Video 1.
CD11c+ cells in islets prevent T cell arrest. NOD.CD11c-DTR BM chimeras containing 90% NOD.CD11c-DTR BM + 10% NOD.CD11c-DTR.8.3.CD2-dsRed BM were treated twice with PBS (intact) or DT (CD11c depleted). 48 h after treatment initiation, T cell motility was analyzed in explanted islets by two-photon microscopy. Representative islet movies from PBS control– or DT-treated mice with 10-min tracks of motion. 8.3 T cells are shown in white, and CD11c-DTR-GFP is shown in green. Dashed line depicts islet border. Time, minutes:seconds; scale bar, 50 µm. 5 frames/sec. Representative of data quantified in Fig. 1, A–G.