Video 2.
Islet-antigen–specific T cells in MERTK−/− islets show decreased motility and increased T cell arrest. Activated BDC-2.5 CD4 T cells were transferred into NOD or NOD.MERTK−/− female mice to initiate islet infiltration. Fluorescently labeled activated 8.3 CD8 T cells (white) and BDC-2.5 CD4 T cells (red) were then transferred into the same mice 3–6 d later. 24 h after fluorescent T cell transfer, islets were isolated and analyzed by two-photon microscopy. Representative islet movies from WT NOD or NOD.MERTK−/− mice with 10-min tracks of motion. Dashed line depicts islet border. Time, minutes:seconds; scale bar, 50 µm. 5 frames/sec. Representative of data quantified in Fig. 3.