Figure S2.

1244 mimotope responses. (A) IL-2 responses (pg/ml) for all mimotopes synthesized based on the unbiased PSL results and dual-defined mixtures, noted as register 1 and register 2. LS-specific 1244c1, 1244c2, and 1244c3 5415 hybridoma cells were stimulated with mimotopes at 2 ng/ml. Data are representative of three independent experiments. (B) Additional mimotopes were synthesized based on the G2D4N5-biased PSL results, and corresponding IL-2 values for each peptide tested at 2 ng/ml with the LS-specific 1244c1, 1244c2, and 1244c3 5415 hybridoma cells are shown. Data are representative of two independent experiments. (C) IL-2 responses for 1244c1 5415 hybridoma cells after stimulation with 1 μg/ml mimotope 46 and 86 with or without 20 μg/ml anti–HLA-DR blocking antibody (L243). Data are representative of two independent experiments. (D) IL-2 responses for the hsp65–specific hybridoma RP15 after stimulation with media or 5 μg/ml of either hsp65 (3–13) of M. leprae, mimotope 46, or mimotope 86. Data are representative of two independent experiments. In A–D, HLA-DR3–expressing B6DK10 fibroblasts were used as APCs, and IL-2 responses were measured by ELISA after a 20–24-h incubation. Mim., mimotope.