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. Author manuscript; available in PMC: 2021 Aug 24.
Published in final edited form as: Cell Rep. 2021 Aug 17;36(7):109556. doi: 10.1016/j.celrep.2021.109556

Figure 2. Increased MT acetylation results in precocious apical insertion.

Figure 2.

(A and B) Side projections of intercalating control and ATAT1 OE MCCs fixed and stained with α-beta tub (A) or α-acetyl. tub (B).

(C and D) Quantification of beta tub (C) and of acetyl. tub (D) in control and ATAT1 OE MCCs. Fluorescence was normalized relative to control (uninjected) MCCs in mosaic embryos for each experiment.

(E) Z-projections displaying progression of MCC apical insertion in control (Tub-GFP) and ATAT1 OE embryos.

(F) Quantification of the percentage of MCCs apically inserted at each stage.

(G and H) Side projections of intercalating control and ATAT1 OE ICs fixed and stained with α-beta tub (G) or α-acetyl. tub (H).

(I and J) Quantification of beta tub (I) and of acetyl. tub. (J) in control and ATAT1 OE ICs. Fluorescence was normalized relative to control (uninjected) ICs in mosaic embryos for each experiment.

(K) Z-projections displaying progression of IC apical insertion in control (Pen-GFP) and Pen-ATAT1 OE embryos.

(L) Quantification of the percentage of ICs apically inserted at each stage.

For all bar graphs (F and L), bars represent the average and error bars indicate SD; for all box-and-whisker plots (C, D, I, and J), the box represents 25%−75% range, the line is the median, and the whiskers represent the total change; and *p < 0.05 and ***p < 0.001.

Analysis includes n > 100 cells from at least 6 embryos per condition (C), n > 80 cells from at least 5 embryos per condition (D), n > 200 cells from at least 9 embryos per condition and ST (F), n > 15 cells from at least 3 embryos per condition (I), n > 50 cells from at least 5 embryos per condition (J), and n > 150 cells from at least 7 embryos per condition and ST (L). Scale bars in (A), (B), (G), and (H) represent 5 μm and in (E) and (L) represent 10 μm.