Antibodies |
|
|
anti-acetylated tubulin (mouse) |
Sigma-Aldrich |
T7451; RRID:AB_609894 |
anti-beta tubulin E7 (mouse) |
Developmental Hybridoma Studies Bank |
RRID: AB_2315513 |
Cy-2-Goat anti-mouse |
Abcam |
Ab6944; RRID: AB_954987 |
Cy-3-Goat anti-mouse |
MilliporeSigma |
AP124CMI; RRID: AB_11213281 |
Cy-5-Goat anti-mouse |
Invitrogen |
A10524; RRID: AB_2534033 |
Chemicals, peptides, and recombinant proteins |
|
|
Alexa Fluor™ Plus 405 Phalloidin |
Invitrogen |
Cat#A30104 |
Phalloidin, DyLight 650 |
Invitrogen |
Cat# PI21838 |
Fluorogel |
Electron Microscopy Sciences |
Cat#1798510 |
nocodazole |
Sigma |
Cat#M1404 |
Critical commercial assays |
|
|
mMESSAGE mMACHINE™ SP6 Transcription Kit |
Invitrogen |
Cat#AM1340 |
RNeasy Mini Kit |
QIAGEN |
Cat#74104 |
Experimental models: Organisms/strains |
|
|
Xenopus laevis (female) |
Nasco |
Cat#LM00535 |
Xenopus laevis (male) |
Nasco |
Cat#LM00715MX |
Xla.Tg(tuba1a:deup1-eGFP)
NXR
|
National Xenopus Resource |
Cat#NXR_0103 |
Xla.Tg(tuba1a:MyrPalm-mRFP1)
NXR
|
National Xenopus Resource |
Cat#NXR_0074 |
Oligonucleotides |
|
|
Morpholino: Control MO: 5′-CCTCTTACCTCAGTTACAATTTATA-3′ |
Gene Tools |
N/A |
Morpholino: ATAT1 MO: 5′-GTGTACGTCAAACTCAAACTCCATG-3′ |
Gene Tools |
N/A |
Primer: ATAT1 for Tub (forward): 5′-GCGAATTCATGGAGTTCCCGTTCGATG-3′ |
This paper |
N/A |
Primer: ATAT1 for Tub (reverse): 5′-GCCTCGAGTTAGTATCGACTCTCCTCAGAG-3′ |
This Paper |
N/A |
Primer: Xmap215 (forward): 5′-GCATCGATATGGGGGATGACAGCG-3′ |
This paper |
N/A |
Primer: Xmap215 (reverse): 5′-GCGAATTCGGCTTTCCGGCTACTTTTG-3′ |
This paper |
N/A |
Primer: HDAC6 (forward): 5′-GCGAGCTCATGACCTCAACCGGCCA-3′ |
This paper |
N/A |
Primer: HDAC6 (reverse): 5′-GCATCGATTAGTGTGGGTGGGGCATA-3′ |
This paper |
N/A |
Primer: ATAT1 for Pen (forward): 5′-GCGGATCCATGGAGTTCCCGTTCGATG-3′ |
This paper |
N/A |
Primer: ATAT1 for Pen (reverse): 5′-GCCTCGAGTTAGTATCGACTCTCCTCAGAG-3′ |
This paper |
N/A |
Recombinant DNA |
|
|
Tub-GFP in pCS2+ vector |
Stubbs et al., 2006
|
N/A |
Tub-RFP in pCS2+ vector |
Stubbs et al., 2006
|
N/A |
Pen-GFP in pCS2+ vector |
Quigley et al., 2011
|
N/A |
Pen-RFP in pCS2+ vector |
Collins et al., 2020a
|
N/A |
GFP-CAMSAP1-Par6 in pCS2+ vector |
Collins et al., 2020a
|
N/A |
Tub-GFP-HDAC6 in pCS2+ vector |
This paper |
modified from Addgene plasmid #13823 |
Tub-GFP-ATAT1 in pCS2+ vector |
This paper |
modified from Addgene plasmid #27099 |
Pen-RFP-ATAT1 in pCS2+vector |
This paper |
modified from Addgene plasmid #27099 |
Tub-XMAP215-GFP in pCS2+ vector |
This paper |
N/A |
Software and algorithms |
|
|
ImageJ |
Schneider et al., 2012
|
https://imagej.nih.gov/ij/
|
NIS-Elements |
Nikon |
https://www.microscope.healthcare.nikon.com/products/software/nis-elements
|