KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-acetylated tubulin (mouse) | Sigma-Aldrich | T7451; RRID:AB_609894 |
| anti-beta tubulin E7 (mouse) | Developmental Hybridoma Studies Bank | RRID: AB_2315513 |
| Cy-2-Goat anti-mouse | Abcam | Ab6944; RRID: AB_954987 |
| Cy-3-Goat anti-mouse | MilliporeSigma | AP124CMI; RRID: AB_11213281 |
| Cy-5-Goat anti-mouse | Invitrogen | A10524; RRID: AB_2534033 |
| Chemicals, peptides, and recombinant proteins | ||
| Alexa Fluor™ Plus 405 Phalloidin | Invitrogen | Cat#A30104 |
| Phalloidin, DyLight 650 | Invitrogen | Cat# PI21838 |
| Fluorogel | Electron Microscopy Sciences | Cat#1798510 |
| nocodazole | Sigma | Cat#M1404 |
| Critical commercial assays | ||
| mMESSAGE mMACHINE™ SP6 Transcription Kit | Invitrogen | Cat#AM1340 |
| RNeasy Mini Kit | QIAGEN | Cat#74104 |
| Experimental models: Organisms/strains | ||
| Xenopus laevis (female) | Nasco | Cat#LM00535 |
| Xenopus laevis (male) | Nasco | Cat#LM00715MX |
| Xla.Tg(tuba1a:deup1-eGFP) NXR | National Xenopus Resource | Cat#NXR_0103 |
| Xla.Tg(tuba1a:MyrPalm-mRFP1) NXR | National Xenopus Resource | Cat#NXR_0074 |
| Oligonucleotides | ||
| Morpholino: Control MO: 5′-CCTCTTACCTCAGTTACAATTTATA-3′ | Gene Tools | N/A |
| Morpholino: ATAT1 MO: 5′-GTGTACGTCAAACTCAAACTCCATG-3′ | Gene Tools | N/A |
| Primer: ATAT1 for Tub (forward): 5′-GCGAATTCATGGAGTTCCCGTTCGATG-3′ | This paper | N/A |
| Primer: ATAT1 for Tub (reverse): 5′-GCCTCGAGTTAGTATCGACTCTCCTCAGAG-3′ | This Paper | N/A |
| Primer: Xmap215 (forward): 5′-GCATCGATATGGGGGATGACAGCG-3′ | This paper | N/A |
| Primer: Xmap215 (reverse): 5′-GCGAATTCGGCTTTCCGGCTACTTTTG-3′ | This paper | N/A |
| Primer: HDAC6 (forward): 5′-GCGAGCTCATGACCTCAACCGGCCA-3′ | This paper | N/A |
| Primer: HDAC6 (reverse): 5′-GCATCGATTAGTGTGGGTGGGGCATA-3′ | This paper | N/A |
| Primer: ATAT1 for Pen (forward): 5′-GCGGATCCATGGAGTTCCCGTTCGATG-3′ | This paper | N/A |
| Primer: ATAT1 for Pen (reverse): 5′-GCCTCGAGTTAGTATCGACTCTCCTCAGAG-3′ | This paper | N/A |
| Recombinant DNA | ||
| Tub-GFP in pCS2+ vector | Stubbs et al., 2006 | N/A |
| Tub-RFP in pCS2+ vector | Stubbs et al., 2006 | N/A |
| Pen-GFP in pCS2+ vector | Quigley et al., 2011 | N/A |
| Pen-RFP in pCS2+ vector | Collins et al., 2020a | N/A |
| GFP-CAMSAP1-Par6 in pCS2+ vector | Collins et al., 2020a | N/A |
| Tub-GFP-HDAC6 in pCS2+ vector | This paper | modified from Addgene plasmid #13823 |
| Tub-GFP-ATAT1 in pCS2+ vector | This paper | modified from Addgene plasmid #27099 |
| Pen-RFP-ATAT1 in pCS2+vector | This paper | modified from Addgene plasmid #27099 |
| Tub-XMAP215-GFP in pCS2+ vector | This paper | N/A |
| Software and algorithms | ||
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| NIS-Elements | Nikon | https://www.microscope.healthcare.nikon.com/products/software/nis-elements |