FIG 3.

FAN1 contributes to MMR efficiency in vivo. The indicated TK6 cell lines were cotransfected with an EGFP control plasmid and an equal amount of a reporter plasmid containing a T/G mismatch in the mCherry gene, as well as a nick in the T-strand. MMR efficiency was estimated from the ratio of the EGFP (green) and the mCherry (red) signal generated by correction of the T/G mismatch to C/G, which converts a TAG stop codon in the mCherry ORF to a TGG Trp codon. The MMR efficiency of the mutants is shown relative to WT cells, which were arbitrarily set to 100%. The results of three independent experiments are shown.