FIG 1.
SNHG20 was upregulated and miR-335 was downregulated in mice with Ang II-induced cardiac hypertrophy. (A) HE staining of heart tissues. (B) Echocardiographic assessment. (C and D) The expression of SNHG20 (C) and miR-335 (D) in heart tissues was detected by qRT-PCR. n = 10 mice per group. (E and F) HL-1 cells were treated with different concentrations of Ang II (10−9, 10−8, 10−7, 10−6, and 10−5 M) for 24 h, and the expression of SNHG20 (E) and miR-335 (F) was detected by qRT-PCR. (G and H) HL-1 cells were treated with 10−6 M Ang II for different times (2, 4, 6, 8, 12, and 24 h), and the expression of SNHG20 (G) and miR-335 (H) was detected by qRT-PCR. Each experiment was repeated three times. *, P < 0.05.