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. 2021 Aug 24;41(9):e00580-20. doi: 10.1128/MCB.00580-20

FIG 2.

FIG 2

Decline of miR-335 by SNHG20. (A) The putative binding site between miR-335 on the 3′-UTR of SNHG20 was predicted by Starbase. (B and C) HL-1 cells were transfected with miR-335 mimics and miR-NC. (B) The expression of miR-335 in HL-1 cells was detected by qRT-PCR. (C) The enrichment of SNHG20 was detected by RNA pulldown assay. (D) HL-1 cells were transfected with sh-SNHG20 and sh-NC. The expression of miR-335 in HL-1 cells was detected by qRT-PCR. (E) HL-1 cells were transfected with pc-SNHG20 and pc-vector. The expression of miR-335 was evaluated by qRT-PCR. Each experiment was repeated three times. *, P < 0.05; **, P < 0.01.