FIG 3.
Cardioprotective role of downregulation of SNHG20 in Ang II-induced fibrosis in vitro. (A) HL-1 cells were transfected with sh-SNHG20 and sh-NC. The expression of SNHG20 in HL-1 cells. (B to E) HL-1 cells were transfected with sh-SNHG20 and sh-NC and then treated with 10−6 M Ang II for 24 h. (B) Cell viability was evaluated by CCK-8 assay. (C) WGA staining in HL-1 cells. (D) Cell size was determined by WGA staining in HL-1 cells. (E) The protein expression of fibronectin, collagen IaI, α-SMA, cleaved PARP, and cleaved aspase-3 in HL-1 cells was detected by Western blotting. Each experiment was repeated three times. *, P < 0.05.