FIG 4.
Effects of SNHG20 in Ang II-induced cardiac fibrosis on HL-1 cells were mediated by miR-335. (A) HL cells were transfected with pc-SNHG20 and sh-NC, and the expression of SNHG20 was evaluated by qRT-PCR. (B to E) HL-1 cells were transfected with sh-SNHG20, or cotransfected with sh-SNHG20 and miR-335 mimics, and then treated with 10−6 M Ang II for 24 h. (B) Cell viability was assessed by CCK-8 assay. (C) WGA staining in HL-1 cells. (D) Cell size was determined by WGA staining in HL-1 cells. (E) The protein expression of fibronectin, collagen IaI, α-SMA, cleaved PARP, and cleaved aspase-3 in HL-1 cells was detected by Western blotting. Each experiment was repeated three times. *, P < 0.05.