Figure 1.

Bmal1 expression is decreased in the distal lower limb muscle of critical limb ischemia (CLI) patients. (A) Relative mRNA expression of Bmal1 in the lower limb muscle of CLI patients detected by real-time PCR. Data are presented as mean ± SEM (n = 9, one-way ANOVA with post-hoc Tukey test). ^*p < 0.05 distal lower limb vs. proximal lower limb. (B,C) Immunohistochemistry conducted in the lower limb muscle of CLI patients with specific anti-BMAL1 antibody with a dilution of 1:100. The immunohistochemical staining results were assigned with a mean score considering the intensity of staining and the proportion of cells with a positive reaction area. Each section was independently assessed by two pathologists without prior knowledge of patient data. Data are presented as mean ± SEM (n = 8, one-way ANOVA with post-hoc Tukey test). ^*p < 0.05 distal lower limb vs. middle lower limb.