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. 2021 Jul 26;17(7):e1009734. doi: 10.1371/journal.ppat.1009734

Fig 7. Fatty acid metabolism in T. congolense.

Fig 7

A) Glucose-derived 13C carbon labelling of saturated fatty acids in T. congolense and T. brucei [45]. Colours correspond to the number of 13C labels detected in each metabolite. B) L-threonine-derived saturated fatty acid 13C labelling in T. congolense. Fatty acid systematic names and numbers: lauric acid: dodecanoic acid, C12:0; myristic acid: tetradecanoic acid, C14:0; palmitic acid: hexadecanoic acid, C16:0; nonadecyclic acid: nonadecanoic acid, C19:0. C) Transcriptomics analysis of acetate and lipid metabolism. Gene names and IDs: Acetate metabolism (PWY1V8-8): AKCT, 2-amino-3-ketobutyrate-CoA ligase, TbTc_6236; TDH, L-threonine 3-dehydrogenase, TbTc_5991; AceCS, acetyl-CoA synthetase, TbTc_0318; PYK1, pyruvate kinase, TbTc_0372; PDHe3, pyruvate dehydrogenase E3, TbTc_4765; PDHe1β, pyruvate dehydrogenase E1 β subunit, TbTc_5437; PPDK, pyruvate phosphate dikinase, TbTc_1304; SCSα, succinyl-CoA synthetase α subunit, TbTc_0813; PDHe1α, pyruvate dehydrogenase E1 α subunit, TbTc_4169; ACH, acetyl-CoA hydrolase/thioesterase, TbTc_5515; PDHe2, dihydrolipoamide acetyltransferase, TbTc_1015. Fatty acid biosynthesis (PWY0-881): ACC, acetyl-CoA carboxylase, TbTc_0754; BKS, β-ketoacyl synthase, TbTc_3372; BKR, β-ketoacyl-ACP reductase, TbTc_1241. Sterol metabolism (PWY1V8-3): SPPS, solanesyl-diphosphate synthase, TbTc_3025; SQase, squalene synthase, TbTc_2577; CYP51A1, lanosterol 14α demethylase, TbTc_4837; SMT, sterol 24-c methyltransferase, TbTc_0387; LSS, lanosteral synthase, TbTc_4540; MVK, mevalonate kinase, TbTc_3761; FPPS, farnesyl pyrophosphate synthase, TbTc_5375; HMGCL, hydroxymethylglutaryl-CoA lyase, TbTc_6160; SM, squalene monooxygenase, TbTc_3357; MDD, mevalonate diphosphate decarboxylase, TbTc_0546; IDI, isopentenyl-diphosphate delta-isomerase, TbTc_1099; PTase, prenyltransferase, TbTc_1352; GGTase-IIβ, geranylgeranyl transferase type II β subunit, TbTc_0680; SCP2, 3-ketoacyl-CoA thiolase, TbTc_4024; PMVK, phosphomevalonate kinase, TbTc_3039; HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase, TbTc_3189. Fatty acid oxidation (FAO-PWY): ECH, enoyl-CoA hydratase, TbTc_3283; ACS3/ACS4, fatty acyl-CoA synthetase 3 & 4, TbTc_0101; ACS2, fatty acyl-CoA synthetase 2, TbTc_0102; LACS5, fatty acyl-CoA synthetase, TbTc_0099; ACSL_0688, long-chain-fatty-acid-CoA ligase, TbTc_0688; ACSL_2381, long-chain-fatty-acid-CoA ligase, TbTc_2381; ACS1, fatty acyl-CoA synthetase 1, TbTc_0100; TFEα1, enoyl-CoA hydratase/enoyl-CoA isomerase, TbTc_3362; ECI_4184, 3,2-trans-enoyl-CoA isomerase, TbTc_4184; SCP2, 3-ketoacyl-CoA thiolase, TbTc_4024; ECI_0360, 3,2-trans-enoyl-CoA isomerase, TbTc_0360; ACAD, acyl-CoA dehydrogenase, TbTc_4954.