Table 1. Comparative analysis of sensitivity to metabolic inhibitors in T. congolense and T. brucei.
Abbreviations: FCCP, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone; SHAM, salicylhydroxamic acid; TAO, trypanosome alternative oxidase; AceCS, acetyl-CoA synthetase.
| Compound | Target | T. congolense EC50 Mean ± SEM | T. brucei EC50 Mean ± SEM | Fold change (Tc/Tb) | P value (t-test) |
|---|---|---|---|---|---|
| Antimycin | Complex III | 271.2 ± 143.5 μM | 144.2 ± 18.1 μM | 1.9 | 0.4295 |
| FCCP | Uncoupling agent | 12.6 ± 5.3 μM | 13.0 ± 5.0 μM | 1.0 | 0.9592 |
| Azide | Complex V (F1-ATPase) | 432.3 ± 127.9 μM | 235.0 ± 6.0 μM | 1.8 | 0.1982 |
| Oligomycin | Complex V (F0-ATPase) | 33.9 ± 14.1 nM | 197.6 ± 39.0 nM | 0.2 | 0.0169 |
| Rotenone | Complex I | 27.4 ± 1.4 μM | 7.4 ± 0.9 μM | 3.7 | 0.0003 |
| SHAM | TAO | 30.22 ± 0.7 μM | 60.23 ± 1.8 μM | 2.0 | 0.0001 |
| SHAM + 10 mM glycerol | TAO | 2.28 ± 3.0 μM | 5.00 ± 0.1 μM | 2.2 | 0.0008 |
| UK5099 | Pyruvate transport | 82.1 ± 8.8 μM | 130.0 ± 5.0 μM | 0.6 | 0.0091 |
| AceCS inhibitor | Acetyl-CoA synthetase | 57.7 ± 15.2 μM | 7.1 ± 2.4 μM | 8.1 | 0.0304 |
| Orlistat | Fatty acid synthase/lipases | 15.6 ± 2.5 μM | 0.02 ± 0.01 μM | 780.0 | 0.0033 |
| Diminazene | Kinetoplast | 50.0 ± 5.6 nM | 32.0 ± 0.5 nM | 1.6 | 0.0425 |