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. 2016 Sep 30;87(2):193–199. doi: 10.2319/020716-107.1

Figure 1.

Figure 1.

Cadherin-11 and β-catenin expressions in PDLCs were suppressed under mechanical stress. (A) Immunofluorescence staining showed that cadherin-11 (red) was expressed in PDLCs and β-catenin (green) located in both cytoplasm and nucleus. Under mechanical stress, the expression of cadherin-11 (red) and β-catenin (green) was suppressed, and β-catenin (green) activity in the nucleus prominently reduced. Scale bars: 20 μm. (B, C) The mRNA and protein expressions of cadherin-11 and protein expression of β-catenin in PDLCs were suppressed with prolonged stress treatment time, examined by real-time PCR (B) and Western blot (C). * P = .05; ** P = .01 versus 0-hour group; # P = .05 ## P = .01 vs 4-hour group; & P = .05, && P = .01 vs 8-hour group. (D, E) The mRNA and protein expressions of cadherin-11 and protein expression of β-catenin in PDLCs were suppressed under mechanical stress with variable intensities, examined by real-time PCR (D) and Western blot (E). ** P = .01 vs 0 g group; * P = .05 vs 0.5-g group.