Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HepG2 | ATCC | HB-8065 RRID:CVCL_0027 |
|
| Cell line (Homo sapiens) | HepG2.2.15 | CCTCC | CCTCC-GDC0141 RRID:CVCL_L855 |
|
| Antibody | Hepatitis B Virus Core Antigen Antibody (1-5) (mouse monoclonal) | ThermoFisher | RRID:AB_1075044 | IF: (1:100) |
| Antibody | Anti-Hepatitis B Virus Core Antigen antibody (rabbit polyclonal) | Abcam | RRID:AB_10903422 | IF: (1:400) |
| Antibody | Hepatitis B Virus Surface Antibody (S 26) (mouse monoclonal) | ThermoFisher | RRID:AB_1075056 | IF: (1:200) |
| Antibody | Anti-ALB/Albumin Antibody (F-10) (mouse monoclonal) | Santa Cruz | sc-271605 RRID:AB_10647230 |
IF: (1:50) |
| Antibody | HNF-4alpha (H-171) antibody (rabbit polyclonal) | Santa Cruz | sc-8987 RRID:AB_2116913 |
IF: (1:50) |
| Antibody | Anti-SLC10A1 antibody produced in rabbit | Sigma-Aldrich | SAB2104761 RRID:AB_10668685 | IF: (1:200) |
| Antibody | Purified Anti-β-Catenin (mouse monoclonal) | BD Transduction Laboratories | 610154 RRID:AB_397555 |
IF: (1:200) |
| Antibody | ANTI-FLAG M2 antibody (mouse monoclonal) | Sigma-Aldrich | F3165 RRID:AB_259529 |
IF: (1:200) |
| Recombinant DNA reagent | pENTR/D-TOPO (plasmid backbone) | ThermoFisher | Invitrogen: K240020 | |
| Recombinant DNA reagent | pLenti6/V5-DEST Gateway Vector (plasmid backbone) | ThermoFisher | Invitrogen: V49610 | |
| Commercial assay or kit | Monolisa HBsAg ULTRA |
Biorad | Biorad: 72346 | |
| Commercial assay or kit | Human Hepatitis B e Antigen (HBeAg) ELISA Kit | Cusabio | CSB-E13557h | |
| Commercial assay or kit | RealiaPrep RNA Cell Miniprep System | Promega | Promega: Z6012 | |
| Commercial assay or kit | DNase I | ThermoFisher | Invitrogen: 18047019 | |
| Commercial assay or kit | SuperScript II Reverse Transcriptase | ThermoFisher | Invitrogen: 18064022 | |
| Commercial assay or kit | GoTaq qPCR Master Mix | Promega | Promega: A6001 | |
| Commercial assay or kit | QIAamp MinElute Virus Spin Kit | Qiagen | Qiagen: 57704 | |
| Commercial assay or kit | DNeasy Blood and Tissue Kits | Qiagen | Qiagen: 69504 | |
| Commercial assay or kit | T5 Exonuclease | New England Biolabs | NEB: M0363 | |
| Commercial assay or kit | Plasmid-Safe ATP-Dependent DNase | Epicentre | E3101K | |
| Chemical compound, drug | Tenofovir disoproxil fumarate | Sigma-Aldrich | SML1794 | |
| Chemical compound, drug | Fialuridine | Cayman Chemical | 15867 | |
| Chemical compound, drug | Myrcludex-B | Creative Peptides | 10-101-139 | |
| Chemical compound, drug | Blasticidine S hydrochloride | Sigma-Aldrich | Sigma: 15205 | |
| Chemical compound, drug | Collagenase D | Sigma-Aldrich | Sigma: 11088858001 | |
| chemical compound, drug | TrypLE Express | ThermoFisher | Gibco:12604013 | |
| Chemical compound, drug | Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear | R and D Systems | 3533-010-02 | |
| Chemical compound, drug | B-27 Supplement, minus vitamin A | ThermoFisher | Gibco: 12587010 | |
| Chemical compound, drug | N-2 Supplement | ThermoFisher | Gibco: 17502048 | |
| Chemical compound, drug | N-Acetyl-L-cysteine | Sigma-Aldrich | Sigma: A7250 | |
| Chemical compound, drug | Nicotinamide | Sigma-Aldrich | Sigma: N3376 | |
| Chemical compound, drug | Recombinant [Leu15]-Gastrin I human | Sigma-Aldrich | Sigma: G9145 | |
| Chemical compound, drug | Recombinant Human EGF | PeproTech | GMP100-15 | |
| Chemical compound, drug | Recombinant Human FGF-10 | PeproTech | 100–26 | |
| Chemical compound, drug | Recombinant Human HGF | PeproTech | 100–39H | |
| Chemical compound, drug | Forskolin | Sigma-Aldrich | Sigma: F3917 | |
| Chemical compound, drug | Recombinant Human Noggin | PeproTech | 120–10C | |
| Chemical compound, drug | A 83–01 | Tocris | Tocris: 2939 | |
| Chemical compound, drug | ROCK Inhibitor (Y-27632) | Sigma-Aldrich | Sigma: SCM075 | |
| Chemical compound, drug | DAPT | Sigma-Aldrich | Sigma: D5942 | |
| Chemical compound, drug | Dexamethasone | Sigma-Aldrich | Sigma: D4902 | |
| Chemical compound, drug | Recombinant Human BMP-7 | PeproTech | 120–03P | |
| Chemical compound, drug | Recombinant Human FGF-19 | PeproTech | 100–32 | |
| Software, algorithm | Code used for RNA-Seq data analysis | Moulos and Hatzis, 2015 | The R package used for the RNA-Seq data analysis is available at https://www.bioconductor.org/packages/release/bioc/html/metaseqR.html | |
| Software, algorithm | GraphPad Prism | https://graphpad.com | RRID:SCR_002798 | Version 5 |