Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Pdgfra-creER™ | JAX #018280 (Rivers et al., 2008) | Tg(Pdgfra-cre/ERT)467Dbe | https://www.jax.org/strain/018280 |
| Strain, strain background (Mus musculus) | R26R | JAX stock #003309 (Soriano, 1994) | Gt(ROSA)26Sortm1Sor | https://www.jax.org/strain/003309 |
| Strain, strain background (Mus musculus) | Ezh2fl | JAX stock #022616 (Shen et al., 2008) | Ezh2tm2Sho | https://www.jax.org/strain/022616 |
| Strain, strain background (Mus musculus) | ConductinlacZ | JAX stock #009120 (Jho et al., 2002; Yu et al., 2005) | Axin2tm1wbm | https://www.jax.org/strain/009120 |
| Strain, strain background (Mus musculus) | RARE-hsp68LacZ | JAX stock #008477 (Rossant et al., 1991) | Tg(RARE-Hspa1b/lacZ)12Jrt | https://www.jax.org/strain/008477 |
| Chemical agent | Tamoxifen | Sigma Aldrich | Cat: T5648 | 10mg/ml in corn oil |
| Chemical agent | BMS453 | Cayman chemical company | Cat: 19076 | 3.5μg/μl in DMSO |
| Chemical agent | all trans-retinoic acid | Sigma Aldrich | Cat: R2625 | 100mg/ml in DMSO |
| Antibody | rabbit anti-Twist2 | Abeam | Cat: ab66031 RRID:AB_2211862 | IF (1:200) |
| Antibody | goat anti-PDGFRα | R&D Systems | Cat: AF1062 RRID:AB_2236897 | IF (1:150) |
| Antibody | rabbit anti-H3K27me3 | Cell Signaling | Cat: 4395 RRID:AB_11220433 | IF (1:1000) |
| Antibody | rabbit anti-Lef1 | Cell Signaling | Cat: 2230, RRID:AB_823558 | IF (1:100) |
| Antibody | goat anti-Dlk1 | R&D Systems | Cat: AF1144 RRID:AB_2230586 | IF (1:50) |
| Antibody | goat anti-Lrig1 | R&D Systems | Cat: AF3688 RRID:AB_2138836 | IF (1:100) |
| Antibody | rabbit anti-TCF4 | Cell Signaling | Cat: 2569 RRID:AB_2199816 | IHC (1:100) |
| Antibody | rabbit anti-TCF1 | Cell Signaling | Cat: 2203 RRID:AB_2199302 | IHC (1:300) |
| Antibody | rabbit anti-K14 | Covance | Cat: PRB-155P RRID:AB_292096 | IF (1:2000) |
| Antibody | rabbit anti-K10 | Covance | Cat: PRB-159P RRID:AB_291580 | IF (1:500) |
| Antibody | rabbit anti-Filaggrin | Covance | Cat: PRB-417P RRID:AB_10064149 | IF (1:500) |
| Antibody | rabbit anti-Sox9 | Millipore | Cat: AB5535 RRID: AB_2239761_ | IF (1:1000) |
| Antibody | and rabbit anti-RARγ | Cell Signaling | Cat: D3A4 RRID:AB_10998934 | IF (1:1000) |
| Antibody | Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor 594 Conjugated | Molecular Probes | Cat: A-11012 RRID:AB_141359 | 1:800 (Secondary antibody) |
| Antibody | Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 | Molecular Probes | Cat: A-21207 RRID:AB_141637 | 1:500 (Secondary antibody) |
| Commercial assay or kit | TSA Cyanine 3 System - antibody amplification kit | Perkin Elmer | Cat: NEL704A001KT RRID:AB_2572409 | Kit |
| Commercial assay or kit | Click-iT Plus EdU kit | Thermo Fisher Scientific | Cat: C10639 | Kit |
| Sequence-based reagent | Ezh2 | Thermo Fisher Scientific | Taqman Assay Mm00468460_ml | qPCR primers Mus musculus |
| Sequence-based reagent | Cyp26b1 | Thermo Fisher Scientific | Taqman Assay Mm00558507_ml | qPCR primers Mus musculus |
| Sequence-based reagent | Twist2 | Thermo Fisher Scientific | Taqman Assay Mm00492147_ml | qPCR primers Mus musculus |
| Sequence-based reagent | Lef1 | Thermo Fisher Scientific | Taqman Assay Mm00550265_ml | qPCR primers Mus musculus |
| Sequence-based reagent | Twist1 | Thermo Fisher Scientific | Taqman Assay Mm00442036_ml | qPCR primers Mus musculus |
| Sequence-based reagent | Axin2 | Thermo Fisher Scientific | Taqman Assay Mm00443610_ml | qPCR primers Mus musculus |
| Sequence-based reagent | Suz12 | Thermo Fisher Scientific | Taqman Assay Mm01304152_ml | qPCR primers Mus musculus |
| Sequence-based reagent | Eed | Thermo Fisher Scientific | Taqman Assay Mm00469660_ml | qPCR primers Mus musculus |
| Sequence-based reagent | Tbx18 | Thermo Fisher Scientific | Taqman Assay Mm00470177_ml | qPCR primers Mus musculus |
| Sequence-based reagent | p75NTR | Thermo Fisher Scientific | Taqman Assay Mm00446296_ml | qPCR primers Mus musculus |
| Sequence-based reagent | Edar | Thermo Fisher Scientific | Taqman Assay Mm00839685_ml | qPCR primers Mus musculus |
| Sequence-based reagent | β-actin | Invitrogen | Cat:4352663 | qPCR primers Mus musculus |
| Software, algorithm | fiji-java6-20170530 | Schindelin et al., 2012 | RRID:SCR_002285 | https://fiji.sc/ |
| Software, algorithm | CellProfiler 3.1.5 | Carpenter et al., 2006 | RRID:SCR_007358 | http://cellnrofiler.org |
| Software, algorithm | Photoshop CC 19.1 | Adobe | RRID:SCR_014199 | https://www.adobe.com/products/photoshop.html |
| Software, algorithm | LAS X Core 3.5.7 | Leica Application Suite X | RRID:SCR_013673) | |
| Software, algorithm | Graphpad Prism v8.4.2 | Graphpad | SCR_002798 |