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Osteogenic differentiation ability of hUC-MSCs in the in vitro two-dimensional culture microenvironment (mean ± SD, n = 6). (a) ALP staining. (b) ALP secretion. (c) Alizarin red staining. (d) Mineralized matrix formation. Scale bar = 100 μm. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
