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Protein secretion related to osteogenic differentiation in hUC-MSCs in the in vitro three-dimensional culture microenvironment (mean ± SD, n = 6). (a) ALP. (b) OCN. (c) BMP-2. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. (d) The hUC-MSCs at passage 3 that were cultured on nHAC/PLA in human MSC serum-free OMD supplemented with 100 ng/ml rhBMP-7 microenvironment exhibited a normal karyotype.
