Table 2.
Effects of anti-TWEAK and anti-TNF monoclonal antibodies on the secretion of IL-8, PGE2 and MMP-1 by dermal fibroblasts stimulated by hTWEAK or TNF
| Stimulus | None | BCB10 (10 μg/ml) | BEB3 (10 μg/ml) | Anti-TNF (10 μg/ml) |
| IL-8 secretion (ng/ml) | ||||
| None | 4.9 ± 0.9 | 5.8 ± 0.6 | 5.9 ± 0.9 | 4.6 ± 0.3 |
| hTWEAK (10 ng/ml) | 19.8 ± 2.8 | 6.0 ± 1.2 | 44.6 ± 6.1 | 18.8 ± 2.6 |
| TNF(1 ng/ml) | 74.3 ± 13 | 109.3 ± 11 | nd | 4.5 ± 0.3 |
| MMP-1 secretion (ng/ml) | ||||
| None | 392 ± 17 | 494 ± 55 | 523 ± 68 | 362 ± 32 |
| hTWEAK (10 ng/ml) | 789 ± 35 | 588 ± 8 | 1287 ± 69 | 827 ± 21 |
| TNF(1 ng/ml) | 2084 ± 154 | 2863 ± 141 | nd | 356 ± 37 |
| PGE2 secretion (ng/ml) | ||||
| None | 33 ± 2 | 24 ± 2 | 90 ± 6 | 44 ± 14 |
| hTWEAK(50 ng/ml) | 47 ± 4 | 25 ± 2 | 103 ± 36 | 52 ± 10 |
| TNF(1 ng/ml) | 190 ± 55 | 212 ± 75 | nd | 31 ± 5 |
| RANTES secretion (ng/ml) | ||||
| None | 0.73 | 0.47 | 0.72 | 0.83 |
| hTWEAK(10 ng/ml) | 17.22 | 1.06 | 47.99 | 24.96 |
| TNF(1 ng/ml) | 1.97 | 2.20 | nd | 0.87 |
| IP-10 secretion (pg/ml) | ||||
| None | 82 ± 10 | 170 ± 40 | nd | 157 ± 34 |
| hTWEAK(10 ng/ml) | 2032 ± 321 | 217 ± 75 | nd | 3274 ± 69 |
| TNF(1 ng/ml) | 331 ± 57 | 253 ± 73 | nd | 78 ± 42 |
Values for IL-8, matrix metalloproteinase-1 (MMP-1), prostaglandin E2 (PGE-2) and interferon-γ-inducible protein-10 (IP-10) are shown as mean ± SD; similar results were obtained in 3 independent experiments. Single analyses for RANTES (regulated on activation, normal T expressed and secreted) were done because the amount of available medium was limited. hTWEAK, human TNF-like weak inducer of apoptosis; nd, not done; TNF, tumour necrosis factor.