Table 3.
Effects of anti-TWEAK and anti-TNF monoclonal antibodies on the secretion of IL-8, PGE2 and MMP-1 by synoviocytes stimulated by hTWEAK or TNF
| Stimulus | None | BCB10 (10 μg/ml) | BEB3 (10 μg/ml) | Anti-TNF (10 μg/ml) |
| IL-8 secretion (ng/ml) | ||||
| None | 55.2 ± 3.1 | 38 ± 1.2 | 117.6 ± 18 | 49 ± 14 |
| hTWEAK | 146.4 ± 17 | 73 ± 19 | 277.2 ± 40 | 113.8 ± 7.3 |
| TNF | 232.6 ± 26 | 245 ± 33 | nd | 44.7 ± 4.8 |
| MMP-1 secretion (ng/ml) | ||||
| None | 4248 ± 276 | 3848 ± 371 | 5383 ± 274 | 3482 ± 584 |
| hTWEAK | 6033 ± 773 | 4381 ± 22 | 7923 ± 1698 | 4878 ± 100 |
| TNF | 6197 ± 210 | 7846 ± 713 | nd | 3505 ± 101 |
| PGE2 secretion (ng/ml) | ||||
| None | 50 ± 12 | 61 ± 6 | 81 ± 13 | 47 ± 13 |
| HTWEAK | 94 ± 7 | 87 ± 26 | 161 ± 30 | 78 ± 3 |
| TNF | 231 ± 49 | 471 ± 79 | nd | 53 ± 5 |
| RANTES secretion (ng/ml) | ||||
| None | 0.12 | 0.23 | 0.23 | 0.05 |
| hTWEAK | 30.78 | 0.26 | 20.12 | 26.01 |
| TNF | 2.19 | 1.17 | nd | 0.05 |
| IP-10 secretion (pg/ml) | ||||
| None | 274 | 386 ± 17 | 349 | 117 ± 84 |
| hTWEAK | 5095 ± 766 | 330 ± 140 | 3158 | 3735 ± 60 |
| TNF | 1710 ± 300 | 1620 ± 20 | nd | 105 |
hTWEAK (TNF-like weak inducer of apoptosis) concentration was 10 ng/ml; TNF (tumour necrosis factor) concentration was 1 ng/ml. Values for IL-8, matrix metalloproteinase-1 (MMP-1), prostaglandin E2 (PGE-2) and interferon-γ-inducible protein-10 (IP-10) are shown as mean ± SD; similar results were obtained in 3 independent experiments. Single analyses for RANTES (regulated on activation, normal T expressed and secreted) were done because the amount of available medium was limited. nd, not done.