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. 2021 Apr 6;19(8):1511–1522. doi: 10.1111/pbi.13566

Figure 4.

Figure 4

OsMESL interacts with OsTrxm in vivo and in vitro. (a) OsMESL interacts with OsTrxm in yeast NMY51. pBT3‐N‐OsMESL and vector pPR3‐N or vector pBT3‐N and pPR3‐N‐OsTrxm as the controls cotransformed into yeast, respectively. (b) OsMESL interacts with OsTrxm in bimolecular luciferase enzyme activity assay. Values are means ± SD (n = 3). (c–d) OsMESL interacts with OsTrxm in a BiFC assay. mCherry, red fluorescence. Chloroplast, white autofluorescence. Scale bars, 5 μm. (d) FLAG tagged OsTrxm was immunopreciptated using an anti‐FLAG antibody and co‐immunopreciptated OsMESL‐GFP was detected by anti‐GFP antibody. (**P ≤ 0.01, Student’s t‐test).