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. 2021 Mar 18;19(8):1553–1566. doi: 10.1111/pbi.13569

Figure 4.

Figure 4

OsRLCK5 regulates RSB resistance. (a) Manhattan plots of loci on chromosome 1 associated with RSB incidence at the seedling, tillering and booting stages at Wenjiang in 2017 (seedling_2017_WJ, tillering_2017_WJ and booting_2017_WJ, respectively). Red markings indicate the strongly associated loci containing the candidate gene OsRLCK5. Horizontal dashed lines indicate the significance threshold (P < 10−6). (b) Local Manhattan plot. The candidate region lies between the red dashed lines. Significant haplotype structures (block 70), P‐values and variant types are indicated on the right side. Dashed line represents the significance threshold (P < 10−6). Red indicated the strongly associated haplotype, which are located within OsRLCK5. (c) Haplotype with three nonsynonymous variations in OsRLCK5 exon. Blue rectangles and black lines indicate exons and introns, respectively. (d) Box plots for RSB resistance, based on the genotypes of two homozygous haplotypes. The horizontal line in the centre of each box denotes the median. The upper and lower limits of each box represent quartiles; whiskers indicate the range of the data. n indicates the number of accessions with the same genotype. (e) Expression analysis of OsRLCK5 in five resistant and five susceptible varieties at 24 hpi by qRT–PCR. (f) Verification of OsRLCK5 expression in resistant and susceptible rice lines at different infection time points by qRT‐PCR. (g) Spatial expression analysis of OsRLCK5 in rice plants. (h) Expression analysis of OsRLCK5 in five resistant and five susceptible varieties at 24h postinoculation (hpi) of R. solani by qRT–PCR. (i) Identification of OsRLCK5‐OE and ‐RNAi lines by qRT–PCR. The rice UBQ gene was used as an internal control. Data are represented as average values with four biological replicates (e–i). (j) Incidence of OsRLCK5‐OE plants was decreased compared with WT, and the incidence of OsRLCK5‐RNAi plants was increased compared with WT. (k) Average incidence rate (n = 10 sheath) of Teqing, OsRLCK5‐RNAi lines, Lemont and OsRLCK5‐OE lines at 4 dpi with R. solani AG1‐IA.