Figure 2. Vδ2⁺γδ T and iNKT-cells are enriched in PN patients.

PBMCs from PN (n=4) and healthy subjects (n=5) (67% African American) were stimulated with PMA and Ionomycin in combination with protein transport inhibitor for 4hrs. The differentiation of T-cell subtypes was assessed by flow cytometry. a, Representative flow cytometry plots for γδ T-cells (CD3⁺CD4⁻ CD8⁻γδTCR⁺ cells). b, Percentage of CD3⁺ γδ T-cells (median ± ICR). c, Representative flow cytometry plots for Vδ1⁺ and Vδ2⁺ subsets of γδ – T cells. d, Percentage of sub-population of γδ-T cells (CD3⁺γδ-TCR⁺Vδ1⁺ or Vδ2⁺) (median ± ICR). e, Representative flow cytometry plots for iNKT-cells (CD3⁺CD4⁻CD8⁻CD56⁺). f, Percentage of CD3⁺ iNKT – cells (median ± ICR). g, Heat map representing geometric mean expression of T-cell populations from healthy and PN subjects as represented by CD4⁺ T helper (CD3⁺CD4⁺CD8⁻) cells, CD8⁺ T cytotoxic cells (CD3⁺CD8⁺ CD4⁻), γδ T-cells (CD3⁺CD4⁻CD8⁻ TCR⁺ cells), iNKT-cells (CD3⁺CD4⁻CD8⁻ CD56⁺). h, Representative flow cytometry plots for CD8 Naïve (CD3⁺CD8⁺CD45RA⁺) cells and CD8 Memory (CD3⁺CD8⁺CD45RO⁺) cells. i, Percentage of CD8 Naïve and CD8 Memory cells (median ± ICR). *P<0.05, healthy controls versus PN subjects, as calculated by a non-parametric Mann Whitney U-test. ns = non-significant.