Table 4.

Summary of stability evaluation for JQ1 in mouse plasma and microdialysates (n=3).

Sample Matrix	Stability Study	Nominal Concentration (ng/mL)	Mean ± S.D. Calculated Concentration (ng/mL)	Precision (% R.S.D.)	Accuracy (% R.E.)	% Mean deviation
Mouse Plasma	Process a	15	14.93 ± 1.10	7.34	−0.46	−3.80
		800	686.39 ± 43.52	6.34	−14.20	−7.24
	Bench-top RT b	15	16.27 ± 1.80	11.07	8.47	4.84
		800	767.80 ± 29.98	3.90	−4.02	3.76
	Bench-top 4C c	15	15.57 ± 0.98	6.30	3.80	0.33
		800	767.45 ± 16.91	2.20	−4.07	3.71
	Freeze-thaw d	15	14.40 ± 0.51	3.52	−3.98	−7.20
		800	793.88 ± 23.31	2.94	−0.77	7.29
	Long-term e	15	14.70 ± 0.69	4.69	−2.03	−5.31
		800	788.56 ± 19.49	2.47	−1.43	6.57
Microdialysate	Process a	1.5	1.45 ± 0.10	6.88	−3.09	0.46
		400	388.38 ± 2.23	0.57	−2.91	1.39
	Bench-top RT b	1.5	1.49 ± 0.06	3.89	−0.42	3.23
		400	403.22 ± 4.61	1.14	0.81	5.26
	Bench-top 4C c	1.5	1.53 ± 0.03	1.84	2.22	5.96
		400	400.64 ± 13.48	3.37	0.16	4.59
	Freeze-thaw d	1.5	1.62 ± 0.017	1.03	7.83	11.77
		400	408.69 ± 6.31	1.54	2.17	6.69
	Long-term f	1.5	1.63 ± 0.15	8.88	8.85	12.83
		400	404.60 ± 6.46	1.60	1.15	5.62

%R.S.D.: relative standard deviation; %R.E.: relative error

^aStability assessed after 48 h in auto sampler at 4°C

^bShort-term stability after 24h at room temperature

^cShort-term stability after 24h at 4°C

^dStability evaluated after three freeze-thaw cycles

^e91 days long-term stability in mouse plasma at −80°C

^f21 days long-term stability in microdialysate at −80°C