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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Kidney Int. 2021 May 27;100(3):672–683. doi: 10.1016/j.kint.2021.04.037

Figure 1. Single-cell RNA-sequencing shows biomarker gene expression in mouse kidney fibroblasts.

Figure 1.

Biomarker gene expression in scRNA-sequencing datasets from animals undergoing sham surgery, or at UUO day 2 (UUO2d), UUO day 7 (UUO7d), or 14 days post R-UUO (RUUO14d) were analyzed. (A) Uniform manifold approximation and projection (UMAP) plot of mouse UUO kidneys reveals 22 separate cell clusters. (B) Biomarker gene expression across cell clusters at different time points. (C) Quantification of differential gene expression at different time points shows that fibrosis biomarkers are primarily expressed in kidney fibroblasts.

UUO, unilateral ureteral obstruction; R-UUO, ureter reimplantation to reverse obstruction; PT1: proximal tubule S1 segment; PT2: proximal tubule S2 segment; PT3: proximal tubule S3 segment; Prolif. PT, proliferating proximal tubule population; PEC, parietal epithelial cells; TAL, thick ascending limb of Loop of Henle; DTL: descending limb of Loop of Henle; DCT, distal convoluted tubule; PC, principal cells; IC, intercalated cells; Fib., fibroblasts type 1; Fib. 2, fibroblasts type 2; aEC, arterial endothelial cells; vEC, venous endothelial cells; gEC, glomerular endothelial cells; Mø: macrophages; DC, dendritic cells; NK cells, natural killer cells. The SERPINF1 (Serine Proteinase Inhibitor-F1) gene encodes PEDF.