Table 2.
HPLC-MS/MS method performance parameters using standard mixtures prepared in mobile phase A.
| Analyte | LOQ (ng/mL) | Linear range (ng/mL) | R2 |
|---|---|---|---|
| GSH | 0.5 | 0.5–1250 | 0.9948 |
| GSSG | 5 | 5–1250 | 0.9987 |
| Cys-Gly | 0.1 | 0.1–50 | 0.9990 |
| Glu-Cys | 0.2 | 0.2–50 | 0.9992 |
| Met | 0.5 | 0.5–50 | 0.9999 |
| MeS | 0.01 | 0.01–50 | 0.9970 |
| Cys | 0.2 | 0.2–50 | 0.9997 |
| Cyss | 0.01 | 0.01–50 | 0.9999 |
| Crt | 1 | 1–50 | 0.9933 |
| Hcys | 0.2 | 0.2–50 | 0.9995 |
| Hcyss | 0.02 | 0.02–50 | 0.9968 |
| NAC | 0.05 | 0.05–50 | 0.9997 |