Table 2.
Real-time PCR primers used in the quantification of rumen micro-organisms.
| Target microflora | Primer sequences (5′ to 3′) | References |
|---|---|---|
| Total bacteria | F: CGGCAACGAGCGCAACCC | Denman and McSweeney (2006) |
| R: CCATTGTAGCACGTGTGTAGCC | ||
| Protozoa | F: GCTTTCGWTGGTAGTGTATT | Sylvester et al. (2004) |
| R: CTTGCCCTCYAATCGTWCT | ||
| Methanogenic archaea | F: TTCGGTGGATCDCARAGRGC | Denman et al. (2007) |
| R: GBARGTCGWAWCCGTAGAATCC | ||
| Fungi | F: AGGAAGTAAAAGTCGTAACAAGGTTTC | Denman and McSweeney (2006) |
| R: CAAATTCACAAAGGGTAGGATGATT | ||
| Ruminococcus albus | F: CCCTAAAAGCAGTCTTAGTTCG | Stevenson and Weimer (2009) |
| R: CCTCCTTGCGGTTAGAACA | ||
| Ruminococcus flavefaciens | F: CGAACGGAGATAATTTGAGTTTACTTAGG | Denman and McSweeney (2006) |
| R: CGGTCTCTGTATGTTATGAGGTATTACC | ||
| Fibrobacter succinogenes | F: GTTCGGAATTACTGGGCGTAAA | Denman and McSweeney (2006) |
| R: CGCCTGCCCCTGAACTATC | ||
| Butyrivibrio fibrisolvens | F: ACCGCATAAGCGCACGGA | Stevenson and Weimer (2009) |
| R: CGGGTCCATCTTGTACCGATAAAT | ||
| Prevotella | F: GGTTCTGAGAGGAAGGTCCCC | Stevenson and Weimer (2009) |
| R: TCCTGCACGCTACTTGGCTG |
F = forward; R = reverse.