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. 2021 Jan 13;82(2):442–458. doi: 10.1007/s00248-020-01672-w

Table 1.

Results of cell cultures on ultrapure agarose, tryptic soy agar, LB agar, and plate count agar plates with water amendments, recording the change in the order of magnitude of CFUs with water amendments (all numbers are scaled by logarithmic transformation and normalized as the difference from the plates without amendments). Positive effects of different volumes of water on microbial growth are in italics, and those caused increase in CFUs more than 1 and 2 orders of magnitude are labeled with + and ++, respectively

Type of culture plate Amendment MES PONR Yungay TZ-0 TZ-4 TZ-5 TZ-6
Ultrapure agarose 1.5 mL H2O − 1.18 0.05 0.55 − 0.89 − 0.32 − 1.15 − 1.08
3 mL H2O − 1.18 0.41 0.39 − 0.60 − 0.27 − 0.97 − 0.63
4.5 mL H2O − 1.18 − 1.11 0.29 − 0.84 − 0.21 − 1.05 − 0.90
Tryptic soy agar 1.5 mL H2O 1.15+ 0.42 − 0.58 − 0.24 − 0.17 − 0.36 − 0.16
3 mL H2O 0.05 − 0.19 1.57+ − 0.25 − 0.30 − 0.05 0.13
4.5 mL H2O 2.20++ 1.80+ 1.83+ − 0.22 1.01+ − 0.41 0.20
LB agar 1.5 mL H2O 0.63 0.29 − 0.41 0.19 − 1.47 − 0.21 0.49
3 mL H2O 0.34 0.53 2.36++ 0.42 − 0.95 0.22 0.32
4.5 mL H2O − 0.36 2.46++ 2.06++ 0.73 0.71 − 0.40 0.45
Plate count agar 1.5 mL H2O 0.65 1.65+ 0.02 − 0.65 − 0.33 0.51 0.71
3 mL H2O 1.10+ 0.13 0.75 − 0.76 − 0.41 0.16 − 0.32
4.5 mL H2O 0.91 2.65++ 0.94 − 0.34 0.29 0.84 0.52