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. 2021 Aug 24;12:5107. doi: 10.1038/s41467-021-25433-6

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© The Author(s) 2021, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Target RNA designs, showing the substituted bases and designed TRM combinations in gray. B Sixteen three-hybrid selections were carried out in duplicate and interaction scores for each RNA in both replicates were plotted. The upper panel of each experiment indicates the locations and substitutions made in the PUF domain and target RNA. The lower panel is a correlation plot between the two replicates, with each dot an RNA sequence. Green indicates the RNA sequence recognized by the wild-type PUF domain; red indicates the target RNA sequence; gray indicates the top two enriched sequences based on the averaged interaction score from the two replicates, if these interaction scores are higher than the target RNA sequence. The X and Y axes indicate two replicates of the experiment. The sequence UCCGACUA was highly enriched in some of the replicates and was removed, and then the interaction scores of the remaining RNA sequences were plotted, which resulted in the different scales of the X and Y axes.