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. 2021 Aug 24;11:17098. doi: 10.1038/s41598-021-96617-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in (A) the relative metallothionein-10 mRNA levels [Type III (stronger) response] and in (B) the relative Hsp70 mRNA levels [Type III (stronger) response], and Hsp72 and 73 levels [Type II & III (faster and stronger) responses] in the mantle of heat hardened (H) and non-hardened (C) Mytilus galloprovincialis when exposed to 24 °C (a,d), 26 °C (b,e) and 28 °C (c,f) compared to control at 18 °C. Representative blots are shown. Blots were quantified using scanning densitometry. Full-length blots/gels are presented in Supplementary Fig. 2. Values are means ± SD, n = 8 preparations from different animals. *p < 0.05 compared to day 0, ⁺p < 0.05 compared to non-hardened (C) mussels.