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. 2021 Aug 24;11:17136. doi: 10.1038/s41598-021-96618-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confirmation of the differential expression of selected proteins using western blot analysis with specific antibodies and densitometry. (a) Western blot analysis using specific antibodies. Pooled myocardial samples (40 μg) were loaded and separated in duplicates. GAPDH was used as a loading control. NCAM-1 (Neural cell adhesion molecule 1), TGM2 (Tissue-type transglutaminase), MAO-A (Monoamine oxidase type A). (b) Quantitative densitometry of the blots confirms more pronounced upregulation of the proteins in RV compared to LV as observed in the proteomic analysis.