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. 2021 Aug 24;6:107. doi: 10.1038/s41541-021-00365-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Overview of vaccination regimen (prime on day 0 and boost on day 28), bleeding time points and groups. Serum samples were collected from mice on a weekly basis. Figure created with BioRender.com. b RBD-specific IgG titer for the groups vaccinated with CuMV_TT control or mCuMV_TT-MERS on days 7, 14, 21, 35, and 49 measured with OD_450nm. c Log₁₀ OD₅₀ values (mean ± SEM) of RBD-specific IgG titer for the groups vaccinated with mCuMV_TT-MERS on days 7,14, 35, and 49 (data from B). d Spike-specific IgG titer for the groups vaccinated with CuMV_TT or mCuMV_TT-MERS on days 7,14,21,35, and 49 measured with OD₄₅₀. e Log₁₀ OD₅₀ values (mean ± SEM) of spike-specific IgG titer for the groups vaccinated with mCuMV_TT-MERS on days 7,14, 35, and 49 (data from D). Statistical analysis using one-way ANOVA. Control group n = 5 and vaccine group n = 5. One representative of 2 similar experiments is shown. The value of p < 0.05 was considered statistically significant (*p < 0.01, **p < 0.001, ***p < 0.0001).