Fig. 5. Induction of high avidity antibodies after vaccination with mCuMV_TT-MERS.

a, d RBD- and spike specific IgG titer for the group vaccinated with mCuMV_TT-MERS vaccine on day 35 measured with OD₄₅₀. After serum incubation one plate was treated with PBS+0.05% Tween 20 and the other plate with 7 M Urea in PBS+0.05% Tween 20. b, e Log₁₀ OD₅₀ values (mean ± SEM) of RBD- and spike-specific IgG titers (shown in a and d) for the group vaccinated with mCuMV_TT-MERS, (p value 0.4860 in b and 0.0327 in e). c, f Avidity index showing the percentage of high avidity RBD- and spike-specific IgG antibodies calculated with the data from (a) or (d). Statistical analysis using Student’s t test. Control group n = 5 and vaccine group n = 5. One representative of 2 similar experiments is shown. The value of p < 0.05 was considered statistically significant (*p < 0.01, **p < 0.001, ***p < 0.0001).